NANOPARTICLES F USE MESOCARP BABASSU (ORBIGNYA PHALERATA MART) AS PLATFORM FOR ENZYMES IN ANCHORAGE BIOSENSORS DEVELOPMENT: A MAPPING TECHNOLOGY
The immobilization of enzymes in the construction of biosensors becomes attractive for enzymatic ability to catalyze very efficiently, biological reactions with high sensitivity and selectivity. The use of nanostructured materials such as polymeric nanoparticles, is an alternative for increasing the efficiency of enzyme immobilization. Thus arises the interest in the synthesis of babassu mesocarp nanoparticles (Orbignya phalerata Mart), because it is a natural, non-toxic and biodegradable biopolymer, easily found in the northeast of the country. Given the reported, this paper proposes a prospective study on the development of a biosensor containing the polyphenol oxidase enzyme and immobilized monolayer by self assembly techniques on the surface of polymeric nanoparticles generated from the babassu mesocarp to sulfites detection in food products, since such compounds are causing allergic reactions in individuals who exhibit sensitivity to this preservative. For scientific and technological mapping, searches were conducted in regular bases Web of Science, Scopus and Scielo, and the banks of patents from the INPI, Espacenet and USPTO, for all patents filed and articles published from 2005 to August 2016. From the articles and patents analyzed, it was verified that the studies related to the babassu mesocarp are scarce, and the development of a biosensor for the detection of sulfite based on polymer nanoparticles of this biopolymer as a platform for the immobilization of the enzyme polyphenol oxidase to Can be quite innovative, since no reports were found in the literature with similar approach.