Volume 11 - Volume 11
Concurrent Down-regulation of the EAF1 and the EAF2 Genes in the Invasive Ductal Carcinoma Type of Breast Cancer
Abstract
In mammals, the EAF1 and EAF2 genes build an active transcription module with other
components. These genes code for factors acting as potent inhibitor of the Wnt-β catenin pathway,
which might be due to their function as tumor suppressor gene. Recently, the involvement of both
above-mentioned factors was described in some human tumors, but not yet in breast cancer. Breast
cancer is one of the most cancer cases in Iran after colon and stomach carcinoma. We aimed
therefore to investigate for the first time a possible correlation between breast cancer and the EAF1
and the EAF2 gene expression.
We collected invasive ductal carcinoma tumor grading (grade 1 to 3) with marginal normal tissue
from forty women diagnosed with breast cancer with the average age of 50 years old. All patients
underwent triple marker test (ER/PR and Her2/neu), indeed the most of them were triple positive
with few triple negative individuals. After RNA extraction, cDNA was synthesized for subsequent
real-time polymerase chain reaction. The humanRPL27 gene wasusedas endogenous control.
Analysis of real-time PCR results showeda significant down-regulation of EAF1 (p-value: 0.028),
and of EAF2 (p-value: 0.0134) in tumor tissue samples in comparison to normal one.There was no
correlation between clinical parameters and the target genes.
We could find significant connection between both tumor suppressor genes in triple positive and
triple negative breast cancer patients, which deserves more attention.
Paper Details
PaperID: 1785
Author's Name: Sogand Heydaran, Sirous Naeimi and Hamid Galehdari
Volume: Volume 11
Issues: Volume 11
Keywords: Breast Cancer, Invasive Ductal Carcinoma, Elongation Factor, EAF1, EAF2.
Year: 2021
Month: April
Pages: 1620-1628